Column headers: qseqid sseqid pident length mismatch gapopen qstart qend sstart send evalue bitscore 1. qseqid query (e.g., gene) sequence id 2. sseqid subject (e.g., reference genome) sequence id 3. pident percentage of identical matches 4. length alignment length 5. mismatch number of mismatches 6. gapopen number of gap openings 7. qstart start of alignment in query 8. qend end of alignment in query 9. sstart start of alignment in subject 10. send end of alignment in subject 11. evalue expect value 12. bitscore bit score